Internalization of VLP-Omicron:(N, E, S, M, M-mCherry & M-pHluorin, T20 RNA) into A549 cells.

A549

A549 cells are lung carcinoma epithelial cells.

No cell labelling,

SASR-CoV-2 VLPs Omicron

SARS-CoV-2 virus-like particles of the Omicron (Omicron BA.1) variant. The particles contain 39 mutations compared to the WT (Wuhan) strain of the virus.

M-mCherry M-pHluorin, Part of the M protein of the particle is tagged with mCherry and part with M-pHluorin. This allows us to track simultaneously the membrane of the particle and the the change of the pH under the microscope.

No treatment